Rabbit Anti-Bovine Serum Albumin (Biotin) Recombinant Antibody

Expression Host： Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Rabbit IgG1

Description：Recombinant, biotinylated rabbit monoclonal antibody against Bovine Serum Albumin protein, produced via Agrobacterium 

                      tumefaciens infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 5 000) ELISA (1: 1 000– 1: 500 000)

Tested Species Reactivity ： Human, Bovine

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.7. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve showing Rabbit Anti-BSA (Biotin) Antibody from 0.00016– 10.0 ng/uL used to detect 200 ng Bovine serum albumin (BSA) antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot analysis showing decreasing amounts of Bovine serum albumin (BSA) (66 kDa) detected with Rabbit Anti-BSA (Biotin) at 1: 1 000 and HRP conjugated Streptavidin at 1: 1000 dilution.