Mouse Anti-CTLA-4 (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length biotinylated Mouse IgG2 recombinant antibody that is specific to CTLA-4. It was produced in

                      Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 40 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity： ≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-CTLA-4 (Biotin) Antibody from 4.0 – 0.000051 ng/uL to detect 25 ng CTLA-4 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of CTLA-4 (~25 kDa) detected with Mouse Anti-CTLA-4 (Biotin) at 1: 1 000 and Streptavidin-HRP conjugated at 1:5 000.