Mouse Anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2 recombinant antibody that is specific to CTLA-4. It was produced in Nicotiana 

                       benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Flow cytometry, ELISA

Dilution Range： Flow Cytometry (1:300-1:500)

ELISA (1: 2 000 – 1: 10 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity： ≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-CTLA-4 to detect coated CTLA-4 Antigen at 0.25 ng/µl and increasing concentrations 

of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Flow cytometry: Peripheral Blood Mononuclear cells (1.3 million cells) were fixed with 4% Paraformaldehyde and washed 2x in PBS buffer. Cells were stained with 1: 400 of Mouse Anti-CTLA-4 Recombinant Antibody, followed by a commercial secondary Anti-Mouse conjugated to Alexa Fluor® 488 at 1: 400 dilution.  Approximately 30 000 events were acquired on a BD FACSDiva 8.0.2 Flow cytometer. A) Dot-plot and B) Histogram of the acquired 30 000 events.