Mouse Anti-CD20 (1F5) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype：Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to the human B-lymphocyte antigen CD20. 

                      It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：ELISA

Dilution Range：ELISA (1: 1 000 – 1: 64 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-CD20 (1F5) to detect coated Human B-lymphocyte antigen CD20 Antigen at 1 ng/µl

and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. ELISA Dose Response curve using Mouse Anti-CD20 (1F5) to detect coated Human B-lymphocyte stored at -20°C and

shipping conditions for two weeks. At shipping the product is placed under conditions that simulate real time shipping conditions. 

Experiments were performed in triplicate, with error bars representing standard deviation (SD).