Human Anti-TNF Alpha (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to TNF Alpha. It was produced in Nicotiana 

                      benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 10 000) ELISA (1: 1 000 – 1: 250 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage： Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：  0.1M Phosphate Buffered Saline, pH 7.4

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1.ELISA Dose Response curve: Human Anti-TNF Alpha (Biotin) Antibody from 0.0002 – 4.0 ng/uL to detect 25 ng TNF Alpha

 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of TNF Alpha (17 kDa) detected with Human Anti-TNF Alpha (Biotin) at 1: 2 000 and

 HRP conjugated Streptavidin at 1: 5 000.