Human Anti-PD-1 (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to PD-1, fused at the genetic level to HRP reporter 

                      enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 2 000) ELISA (1: 1 000 – 1: 256 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4 Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-PD-1 (HRP) Antibody from 0.02 – 50 ng/uL to detect 50 ng Human PD1/ PDCD1 

Protein (His tag). Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of human PD-1 protein (17.4 kDa, migrates at ~41.1 kDa due to glycosylation) detected 

with Human Anti-PD-1 (HRP) Antibody at 1: 2 500.