Human Anti-Interleukin-8 (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to Interleukin 8 and conjugated to Biotin. 

                      It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 10 000)ELISA (1: 1 000 – 1: 60 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1M Phosphate Buffered Saline, pH 7.4

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: Human Anti-IL-8 Antibody from 4.0– 0,00024 ng/uL used to detect 10 ng IL-8 (Human IL-8/CXCL8 

Protein (His tag) antigen). Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human IL-8/CXCL8 Protein (His tag) (12 kDa) detected with Human Anti-IL-8

 (Biotin) at 1: 5 000 and Streptavidin-HRP at 1: 5 000.