Human Anti-Interleukin-2 (1F90) (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to Interleukin 2 (IL-2), fused at the genetic 

                      level to the reporter enzyme HRP. It was produced in Nicotiana benthamiana plants via Agrobacterium 

                      tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：ELISA (1: 1 000 – 1: 20 000)Western blot (1: 1 000 – 1: 3 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1M Phosphate Buffered Saline, pH 7.4 Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: Human Anti-IL-2 (HRP) Antibody from 0.0006 – 50 ng/uL to detect 100 ng Interleukin 2 protein.

 Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot showing decreasing amounts of Interleukin 2 (IL-2) 16.9 kDa (migrates as 2 bands at ~18.4 kDa and 16.6 kDa

 due to glycosylation) detected with Human Anti-IL-2 (HRP) Antibody at 1: 1 000.