Human Anti-Alpha Tubulin (F2C) (HRP)

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description： This product is a full-length Human IgG1 antibody specific to Human Alpha tubulin protein, fused at the genetic

                       level to the HRP reporter enzyme. It was produced recombinantly in Nicotiana benthamiana plants via Agrobacterium

                       tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1000 – 1: 4000)

ELISA (1: 500 – 1: 200 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4 Preservative: None.

Purification Notes： This product was purified using Protein A affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-Alpha Tubulin (HRP) Antibody from 2.0 – 0,0000256 ng/uL to detect 25 ng 

TUBA1A antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human Alpha (α) Tubulin (~50kDa) detected with Human Anti-Alpha Tubulin (HRP) at 1: 4 000.