Human Anti-SARS CoV-2 Nucleocapsid Protein (HuN5) (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, fused at the genetic level to HRP. This protein 

                      was produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1000 – 1: 2 000) ELISA (1: 1 000 – 1: 64 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4.Preservative: none

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE.

98.71 % as determined by Mass Spectrometry.

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1: ELISA Dose Response curve using Human Anti-N (HuN5) (HRP) Antibody from 0,00064– 50 ng/uL to detect 50 ng SARS-CoV-2 Nucleocapsid protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2: Western blot analysis of decreasing amounts of SARS-CoV-2 N Protein (46 kDa) detected with Human Anti-N (HuN5) (HRP) Antibody at 1: 1 000.