Human Anti-SARS CoV-2 Nucleocapsid Protein (HuN4) (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, fused at the genetic level to HRP. This protein 

                      was produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 5 000) ELISA (1: 1000 – 1: 1 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4. Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography..

Purity：≥ 95 % as determined by SDS-PAGE.

98.66 % as determined by Mass Spectrometry.

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1: ELISA Dose Response curve using Human Anti-N (HuN4) (HRP) Antibody from 0,00006 – 1.0 ng/uL to detect 50 ng recombinant SARS-CoV-2 Nucleocapsid protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2: Western blot analysis of decreasing amounts of SARS-CoV-2 Nucleocapsid phosphoprotein (~46 kDa) detected with Human Anti-N (HuN4) (HRP) at 1: 1 000. No secondary antibody is required.