Mouse Anti-SARS CoV-2 Nucleocapsid Protein (N12.8) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：Recombinant mouse monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, produced via Agrobacterium tumefaciens 

                      infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 2 000) ELISA (1: 1 000 – 1: 4 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage： Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4.

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE

94.20% as determined by Mass Spectrometry.

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of Mouse Anti-N (N12.8) detecting 1 ng/µl SARS-CoV-2 Nucleocapsid Phosphoprotein.

Figure 2. Western blot analysis of Mouse Anti-N (N12.8). Lanes 1 – 4: Varying amounts of SARS-CoV-2 Nucleocapsid (N) Phosphoprotein were run on the SDS-PAGE. Separated bands were transferred to the membrane and Mouse Anti-N (N12.8) (1: 1 000) was used to detect the antigen. The bands SARS-CoV-2 Nucleocapsid (N) Phosphoprotein (~46 kDa) were visualized in each lane following the addition of anti-mouse secondary antibody with HRP and substrate. Post translation cleavage of the N protein is seen at ~40kDa. Our antibody was able to detect antigen amounts upwards of 12.5 ng (Lane 3).