Rabbit Anti-Hemagglutinin Tag Recombinant Antibody

Expression Host： Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Rabbit IgG1

Description： This product is a full-length Rabbit IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag). It was produced in Nicotiana 

                       benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Target Name：Hemagglutinin Tag (HA Tag) (HA tag is derived from the HA-molecule (P03437-HEMA_I68A0) corresponding to amino acids 98-106).

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 2 000) ELISA (1: 1 000 – 1: 160 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.8. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Rabbit Anti-HA Tag Antibody from 120 – 0,08 ng/uL to detect 70 ng HA peptide antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot:decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with Rabbit Anti-HA Tag at 1: 1 000 and an HRP conjugated anti-rabbit secondary antibody.