Rabbit Anti-Cortisol (5A4) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Rabbit IgG1

Description：This product is a full-length Rabbit IgG1 recombinant antibody specific to Cortisol. It was produced in Nicotiana benthamiana plants 

                       via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 20 000)

ELISA (1: 2 000 – 1: 2 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.8. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Rabbit Anti-Cortisol (5A4) Antibody from 0,00003 – 0,5 ng/uL to detect 25 ng BSA-Cortisol antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western Blot of decreasing amounts of BSA-Cortisol (~90 kDa) detected with Rabbit Anti-Cortisol (5A4) Antibody at 1: 20 000 and an HRP conjugated Anti-Rabbit secondary antibody.