Rabbit Anti-c-Myc (9E10) (eGFP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Rabbit IgG1

Description：This product is a full-length Rabbit IgG1 recombinant antibody specific to c-Myc and fused at the genetic level to eGFP. It was 

                      produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA, fluorescent western blot

Dilution Range： Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 512 000)

Fluorescent western blot (1: 1 000 – 1: 2 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.8. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: Rabbit Anti-c-Myc (eGFP) Antibody from 0.000032– 2.0 ng/uL to detect 10 ng Human Serglycin/SRGN Protein (His & Myc Tag) antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of Human Serglycin/SRGN Protein (c-Myc Tagged) (20 kDa) detected with Rabbit Anti-c-Myc (eGFP) at 1: 1 000 and an HRP conjugated anti-Rabbit secondary antibody at 1: 10 000.