Mouse Anti-Vascular Endothelial Growth Factor (VEGF) (Biotin) Recombinant Antibody

Expression Host： Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Vascular Endothelial Growth Factor (VEGF-165a). It was 

                      produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： ELISA (1: 1 000 – 1: 10 000) Western blot (1: 1 000 – 1: 2 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.8. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-VEGF (Biotin) Antibody from 4.0– 0.00005 ng/uL to detect 50 ng Recombinant Human VEGF antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Recombinant Human VEGF (~28 kDa) detected with Mouse Anti-VEGF (Biotin) at 1: 1 000 and HRP conjugated Streptavidin at 1: 5 000.