Mouse Anti-Vascular Endothelial Growth Factor Recombinant Antibody

Expression Host： Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG4a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to the VEGF-165a antigen. It was produced in Nicotiana 

                       benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 5 000-1: 10 000) ELISA (1: 20 000-1: 100 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.7. Preservative: None

Purification Notes：This product was purified using Protein A- affinity chromatography.

Purity：≥ 90% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve of Mouse Anti-VEGF Recombinant Antibody used to detect coated VEGF antigen at 0.05 ng/µl and increasing amount of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2: Western blot of decreasing amounts of commercial recombinant VEGF (17 kDa) detected using Mouse Anti-VEGF at 1: 2 000 and an HRP conjugated anti-mouse secondary antibody.