Mouse Anti-Tumour Necrosis Factor Alpha Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to TNF-α. It was produced in Nicotiana benthamiana plants 

                       via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western Blot (1: 2 000-1: 10 000), ELISA (1: 1 000 – 1: 10 000),

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.7. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-TNF-α from 2 – 0.00012207 ng/uL to detect 0.1 ng TNF-α antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of TNF-α 17.5 kDa detected with Mouse Anti-TNF-α at 1: 5 000 and an HRP conjugated anti-mouse secondary antibody.