Mouse Anti-Interleukin-8 (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Interleukin 8 (IL-8). It was produced in Nicotiana 

                       benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 10 000) ELISA (1: 1 000 – 1: 100 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.6. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-IL-8 (Biotin) Antibody from 4.0 – 0,00024 ng/uL to detect 10 ng IL-8 (Human IL-8/CXCL8 Protein (His tag) antigen. Experiments were performed in duplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human IL-8/CXCL8 Protein (His tag) (12 kDa) detected with Mouse Anti-IL-8 (Biotin) at 1: 5 000 and Streptavidin-HRP at 1: 5 000.