Mouse Anti-Interleukin-8 Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG3a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Interleukin 8 (also known as CXCL8) Antigen. 

                      It was produced in Nicotiana benthamiana plants via

Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： WB (1: 5 000 – 1: 10 000) ELISA (1: 30 000 – 1: 1 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage： Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.6. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 90% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-IL-8 to detect coated IL-8 Antigen at 0,25 ng/µl and increasing concentrations of 

antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Functionality western blot analysis using Mouse Anti-IL-8 (1: 10 000) to detect decreasing amounts of IL-8 Antigen (~12 kDa) and

 detected using an Anti-Mouse secondary conjugated to HRP at 1: 10 000.