Mouse Anti-HA Tag (12CA5) (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to the HA Tag. It was produced in

                      Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA, fluorescent western blot

Dilution Range：  Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 10 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: Mouse Anti-HA Tag (Biotin) Antibody from 0.00024– 4.0 ng/uL used to detect 25 ng HA Peptide. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with Mouse Anti-HA Tag (Biotin) at 1: 1 000 and an HRP conjugated streptavidin secondary antibody.