Mouse Anti-Glutathione S-transferase Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2 recombinant antibody specific to the GST Tag. It was produced in Nicotiana 

                      benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 5 000 – 1: 50 000)

ELISA (1: 2 000 – 1: 50 000)

Tested Species Reactivity ： Human

Concentration ：0.5 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-GST to detect coated GST Antigen at 0.05 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of GST antigen (28 kDa) detected with Mouse Anti-GST Recombinant Antibody at 1:5 000 and an HRP conjugated anti-mouse secondary antibody.