Mouse Anti-Delta-9-THC (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Delta-9-THC. It was produced in Nicotiana

                      benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 65 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: using Mouse Anti-Delta-9-THC (Biotin) Antibody from 32– 0,00024 ng/uL to detect 50 ng THC-BSA antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of THC-BSA protein (monomer: 73 kDa, dimer at 140 kDa and tetramer at 260/280 kDa) detected with Mouse Anti-Delta-9-THC (Biotin) at 1: 1 000 and Streptavidin-HRP conjugated at 1/5000.