Mouse Anti-CD28 Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to the CD28 antigen. It was produced in Nicotiana 

                      benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：  Western blot (1: 1 000 – 1: 6 000), ELISA (1: 500 – 1: 8 000)

Tested Species Reactivity ：Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-CD28 from 4 – 0.0002 ng/µl to detect 25 ng CD28 antigen. Experiments were 

performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of CD28 Antigen (35-50 kDa) detected with Mouse Anti-CD28 at 1: 2 000 and an HRP 

conjugated anti-mouse secondary antibody at 1: 10 000.

Figure 3. ELISA Dose Response curve detecting 25 ng CD28 antigen using Mouse Anti-CD28 Antibody from 4 – 0.0002 ng/µl stored 

at -20°C and shipping conditions for two weeks. For shipping conditions, the product is placed under conditions that simulate real 

time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).