Mouse Anti-Beta Tubulin (S11B) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype：Mouse IgG2a

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Beta Tubulin. It was produced in Nicotiana

                      benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA, Immunocytochemistry

Dilution Range：Western blot (1: 5 000 – 1: 10 000)

ELISA (1: 65 000 – 1: 1 000 000)

Immunocytochemistry (1:100-1:300)

Tested Species Reactivity ： Human, Mouse

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-Beta Tubulin (S11B) to detect coated Recombinant Human Tubulin beta chain 

Antigen at 0.25 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing 

standard deviation (SD).

Figure 2. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. 

Following fixation and blocking, cells were incubated with 1:200 dilution of Mouse Anti-β-Tubulin primary antibody, and 1:300 dilution

of a commercial Anti-Mouse Alexa Fluor® 488 conjugated commercial secondary antibody. Images were taken on a Zeiss LSM780 with

ELYRA PS1 platform confocal microscope (20X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for 

kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

Figure 3. Western blot of decreasing amounts of recombinant Human beta chain antigen (50-70 kDa) detected with Mouse Anti-Beta 

Tubulin (S11B) Antibody at 1: 10 000 and detected with a HRP conjugated anti-mouse secondary antibody at 1: 10 000.