Human Anti-VEGF (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to VEGF, fused to HRP at the genetic level. 

                      It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： ELISA (1: 1 000 – 1: 520 000)Western blot (1: 1 000 – 1: 2 000)

Tested Species Reactivity ： Human

Concentration ： 1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4 Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Figure 1. ELISA Dose Response curve using Human Anti-VEGF (HRP) Antibody from 0.0098 – 40.0 ng/uL to detect 3.0 ng recombinant

 VEGF antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Recombinant Human Vascular Endothelial Growth Factor (VEGF) (~30 kDa isoform) 

detected with Human Anti-VEGF (HRP) Antibody at 1: 1 000.