Human Anti-Interleukin-8 Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to Interleukin 8 (also known as CXCL8) Antigen.

                      It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 5 000)ELISA (1: 15 000 – 1: 1 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1M Phosphate Buffered Saline, pH 7.4

Purification Notes：This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-IL-8 to detect coated IL-8 Antigen at 1 ng/µl and increasing concentrations 

of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Functionality western blot analysis using Human Anti-IL-8 (1: 5 000) to detect decreasing amounts of IL-8 Antigen (~12 kDa) 

and detected using an Anti-Human secondary conjugated to HRP at 1: 5 000.