Human Anti-Interleukin-2 (1F90) (Biotin) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to Interleukin 2 (IL-2). It was produced in 

                      Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：ELISA (1: 1 000 – 1: 10 000) Western blot ((1: 1 000 – 1: 2 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1M Phosphate Buffered Saline, pH 7.4

Purification Notes：This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve:Human Anti-IL-2 (1F90) (Biotin) Antibody from 40– 0,002 ng/uL to detect 25 ng Human IL-2 antigen. 

Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Interleukin-2 (17 kDa) detected with Human Anti-IL-2 (1F90) (Biotin) at 1:1 000 

and Streptavidin-HRP conjugated at 1:5 000.