Human Anti-HA Tag (12CA5) (HRP)

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype：Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag) and fused at the 

                      genetic level to HRP. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 160 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1M Phosphate Buffered Saline, pH 7.4 Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-HA Tag (12CA5) (HRP) Antibody from 40– 0,008 ng/uL to detect 10 ng HA peptide

 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: Decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with Human Anti-HA Tag

 (12CA5) (HRP) at 1: 1 000.