Human Anti-CTLA-4 Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to CD28. It was produced in Nicotiana 

                      benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：ELISA (1: 2 000 – 1: 50 000)

Western blot (1: 1 000 – 1: 2 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1M Phosphate Buffered Saline, pH 7.4

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-CTLA-4 Antibody from 0.5 – 0.0000305 ng/uL to detect 10 ng CTLA-4 antigen. 

Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of CTLA-4 (22-27 kDa) detected with Human Anti-CTLA-4 Antibody at 1:1 000 and an

 HRP conjugated anti-human secondary antibody.