Human Anti-c-Myc (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 recombinant antibody specific to c-Myc and fused at the genetic level to HRP. 

                      It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 250 – 1: 100 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：   0.1  M Phosphate Buffered Saline, pH 7.4 Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: Human Anti-c-Myc (HRP) Antibody from 0.000977– 16 ng/uL to detect 15 ng Human Serglycin/SRGN 

Protein (His & Myc Tag) antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of Human Serglycin/SRGN Protein (His & Myc Tag) (~20 kDa) detected with Human Anti-c-Myc (HRP) Antibody at 1: 2 000.