Human Anti-Alpha Tubulin (F2C) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：This product is a full-length Human IgG1 antibody specific to Human Alpha tubulin protein. It was produced 

                      recombinantly in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration

Verified Applications：Western blot, ELISA.

Dilution Range：Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 1 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4 Preservative: None

Purification Notes： This product was purified using Protein A affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Human Anti-Alpha Tubulin (F2C) to detect coated Human Alpha Tubulin Antigen 

at 1 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing 

standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human Alpha tubulin antigen (~50 kDa) detected using Human Anti-Alpha 

Tubulin (F2C) at 1: 5 000.