Rabbit Anti-GST Biotinylated Recombinant Antibody

Expression Host： Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Rabbit IgG1

Description：This product is a full-length biotinylated Rabbit IgG1 recombinant antibody specific to Glutathione-S-Transferase (GST). It was 

                      produced in  Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 1 000 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.7. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.  

Image：

Figure 1. ELISA Dose Response curve: Rabbit Anti-GST (Biotin) Antibody from 0.0002– 4.0 ng/uL to detect 25 ng GST antigen. Experiments 

were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of GST protein (28 kDa) detected with Rabbit Anti-GST (Biotin) Antibody at 1: 10 000 and HRP

 conjugated Streptavidin secondary antibody at 1: 5 000.