Rabbit Anti-GST Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype：Rabbit IgG1

Description：This product is a full-length Rabbit IgG1 recombinant antibody specific to the GST Tag. It was produced in Nicotiana

                      benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 5 000-1: 10 000)

ELISA (1: 20 000-1: 100 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4

Preservative: None

Purification Notes： This product was purified using Protein A affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE

98.94% as determined by Mass Spectrometry

General Notes： For Research Use only, unless otherwise indicated.  

Image：

Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of Rabbit Anti-GST added to a 96 well

 plate coated with 0.1 ng/µl GST antigen.

Figure 2. Western blot of decreasing amounts of GST antigen (28 kDa) detected with Rabbit Anti-GST at 1: 5 000 and an HRP 

conjugated anti-rabbit secondary antibody.