Human Anti-Tumour Necrosis Factor Alpha Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：Recombinant human monoclonal antibody against TNF-Alpha cytokine, produced via Agrobacterium tumefaciens

                     infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 2 000 – 1: 20 000)

ELISA (1: 1 000 – 1: 10 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4.

Purification Notes：This product was purified using Protein A affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE.

98.28 % as determined by Mass Spectrometry.

General Notes：  For Research Use only, unless otherwise indicated.

Image：

Figure 1: Functionality Testing of Human Anti-TNF-Alpha ELISA Dose Response curve showing increasing absorbance with increasing amounts 

96 well plate coated with 0.4 ng/µl of TNF alpha antigen.

Figure 2:  Western blot analysis of Human Anti-TNF-Alpha. Lanes 2 – 6: Varying amounts of TNF- Alpha were run on an 

SDS-PAGE gel. Separated bands were transferred to the membrane and Human Anti-TNF-α (1: 5 000) was used to detect

the antigen. The single band of TNF-Alpha cytokine was visualized following the addition of anti-human secondary 

antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 25 ng (Lane 5).